Nutritional and environmental regulation of highly unsaturated fatty acid synthesis and fatty acid oxidation in Atlantic salmon (Salmo salar L.) enterocytes and hepatocytes
نویسندگان
چکیده
Running title: PUFA metabolism in salmon fed vegetable oil
منابع مشابه
Environmental and dietary influences on highly unsaturated fatty acid biosynthesis and expression of fatty acyl desaturase and elongase genes in liver of Atlantic salmon (Salmo salar).
Highly unsaturated fatty acid (HUFA) synthesis in Atlantic salmon (Salmo salar) was known to be influenced by both nutritional and environmental factors. Here we aimed to test the hypothesis that both these effectors involved similar molecular mechanisms. Thus, HUFA biosynthetic activity and the expression of fatty acyl desaturase and elongase genes were determined at various points during an e...
متن کاملHighly Unsaturated Fatty Acid Synthesis in Vertebrates: New Insights with the Cloning and Characterisation of a ∆6 Desaturase of Atlantic Salmon
Fish are an important source of the n-3 highly unsaturated fatty acids (HUFA), 1 eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids that are crucial to the health of higher 2 vertebrates. The synthesis of HUFA involves enzyme-mediated desaturation, and a ∆5 fatty acyl 3 desaturase cDNA has been cloned from Atlantic salmon (Salmo salar) and functionally 4 characterized previously. Here we re...
متن کاملFatty acid metabolism in Atlantic salmon (Salmo salar L.) hepatocytes and influence of dietary vegetable oil.
Isolated hepatocytes from Atlantic salmon (Salmo salar), fed diets containing either 100% fish oil or a vegetable oil blend replacing 75% of the fish oil, were incubated with a range of seven (14)C-labelled fatty acids. The fatty acids were [1-(14)C]16:0, [1-(14)C]18:1n-9, 91-(14)C]18:2n-6, [1-(14)C]18:3n-3, [1-(14)C]20:4n-6, [1-(14)C]20:5n-3, and [1-(14)C]22:6n-3. After 2 h of incubation, the ...
متن کاملEffects of diets containing vegetable oil on expression of genes involved in highly unsaturated fatty acid biosynthesis in liver of Atlantic salmon (Salmo salar)
Running title: HUFA gene expression in salmon fed vegetable oil Abstract Fish are an important dietary source of the long-chain C 20 and C 22 highly unsaturated fatty acids (HUFA), arachidonate (20:4n-6), eicosapentaenoate (20:5n-3) and docosahexaenoate (22:6n-3), that are crucial to the health of higher vertebrates and that can be beneficial in human diets. Δ5 and Δ6 fatty acid desaturases, an...
متن کاملEffects of a high linoleic acid diet on the fatty acid composition of leucocytes from Atlantic salmon (Salmo salar).
Mammalian cells can metabolize linoleic acid (C,8:2, , , , , ) by A-6-desaturation to y-linoleic acid (C18:3,,,-,,), which can be elongated to dihomo-y-linoleic acid (C211.3,,z, ), and subsequently undergo A-5-desaturation to arachidonic acid (AA) (C211:J,rr-,,) 11 I . Most freshwater fish are also capable o f this process 121, whereas marine species such as turbot (Scopthulmirs maximus) appare...
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